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bactrox hypoxic chamber  (Sheldon Manufacturing)

 
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    Structured Review

    Sheldon Manufacturing bactrox hypoxic chamber
    Light microscopy images of clinical S. aureus isolate <t>A1</t> cultured in SSF without Fg (SSF1) and SSF with Fg (SSF2) under aerobic ( A ), microaerophilic (3% O 2 ) ( B ), and anaerobic ( C ) conditions, evaluated at four different timepoints. The bars represent the average number ( n = 3) of CFUs recovered after conventional biofilm disruption for biofilms grown in SSF1 (blue) and SSF2 (light gray) or after biofilm disruption using trypsin (0.25%) after growth in SSF2 (dark gray). Error bars indicate standard deviation; black line indicates the inoculum of 5 × 10 7 CFU/mL. * P < 0.05, ** P < 0.01, *** P < 0.001. ns, not significant ( P ≥ 0.05).
    Bactrox Hypoxic Chamber, supplied by Sheldon Manufacturing, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bactrox hypoxic chamber/product/Sheldon Manufacturing
    Average 90 stars, based on 1 article reviews
    bactrox hypoxic chamber - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "A novel synthetic synovial fluid model for investigating biofilm formation and antibiotic susceptibility in prosthetic joint infections"

    Article Title: A novel synthetic synovial fluid model for investigating biofilm formation and antibiotic susceptibility in prosthetic joint infections

    Journal: Microbiology Spectrum

    doi: 10.1128/spectrum.01980-24

    Light microscopy images of clinical S. aureus isolate A1 cultured in SSF without Fg (SSF1) and SSF with Fg (SSF2) under aerobic ( A ), microaerophilic (3% O 2 ) ( B ), and anaerobic ( C ) conditions, evaluated at four different timepoints. The bars represent the average number ( n = 3) of CFUs recovered after conventional biofilm disruption for biofilms grown in SSF1 (blue) and SSF2 (light gray) or after biofilm disruption using trypsin (0.25%) after growth in SSF2 (dark gray). Error bars indicate standard deviation; black line indicates the inoculum of 5 × 10 7 CFU/mL. * P < 0.05, ** P < 0.01, *** P < 0.001. ns, not significant ( P ≥ 0.05).
    Figure Legend Snippet: Light microscopy images of clinical S. aureus isolate A1 cultured in SSF without Fg (SSF1) and SSF with Fg (SSF2) under aerobic ( A ), microaerophilic (3% O 2 ) ( B ), and anaerobic ( C ) conditions, evaluated at four different timepoints. The bars represent the average number ( n = 3) of CFUs recovered after conventional biofilm disruption for biofilms grown in SSF1 (blue) and SSF2 (light gray) or after biofilm disruption using trypsin (0.25%) after growth in SSF2 (dark gray). Error bars indicate standard deviation; black line indicates the inoculum of 5 × 10 7 CFU/mL. * P < 0.05, ** P < 0.01, *** P < 0.001. ns, not significant ( P ≥ 0.05).

    Techniques Used: Light Microscopy, Cell Culture, Disruption, Standard Deviation

    CLSM (left) and light microscopy (right) images of four of the staphylococci. ( A ) S. aureus A1, ( B ) S. aureus SAU060112, ( C ) S. capitis subsp. capitis CCUG 39451, and ( D ) S. epidermidis HD05-1 ST2, after 24 h of incubation in SSF2. CLSM: scale bars 50 µm; light microscopy: scale bars 200 µm. The contrast was adjusted to improve visualization. The light microscopy images shown (on the right) are the same as the ones shown in for the four strains mentioned.
    Figure Legend Snippet: CLSM (left) and light microscopy (right) images of four of the staphylococci. ( A ) S. aureus A1, ( B ) S. aureus SAU060112, ( C ) S. capitis subsp. capitis CCUG 39451, and ( D ) S. epidermidis HD05-1 ST2, after 24 h of incubation in SSF2. CLSM: scale bars 50 µm; light microscopy: scale bars 200 µm. The contrast was adjusted to improve visualization. The light microscopy images shown (on the right) are the same as the ones shown in for the four strains mentioned.

    Techniques Used: Light Microscopy, Incubation

    Overview of median MICs, MBCs, BPCs, and MBICs (in µg/mL) of the different antibiotics for the 18 clinical PJI isolates investigated <xref ref-type= a " title="Overview of median MICs, MBCs, BPCs, and MBICs (in µg/mL) of the different ... " property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: Overview of median MICs, MBCs, BPCs, and MBICs (in µg/mL) of the different antibiotics for the 18 clinical PJI isolates investigated a

    Techniques Used:



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    Sheldon Manufacturing bactrox hypoxic chamber
    Light microscopy images of clinical S. aureus isolate <t>A1</t> cultured in SSF without Fg (SSF1) and SSF with Fg (SSF2) under aerobic ( A ), microaerophilic (3% O 2 ) ( B ), and anaerobic ( C ) conditions, evaluated at four different timepoints. The bars represent the average number ( n = 3) of CFUs recovered after conventional biofilm disruption for biofilms grown in SSF1 (blue) and SSF2 (light gray) or after biofilm disruption using trypsin (0.25%) after growth in SSF2 (dark gray). Error bars indicate standard deviation; black line indicates the inoculum of 5 × 10 7 CFU/mL. * P < 0.05, ** P < 0.01, *** P < 0.001. ns, not significant ( P ≥ 0.05).
    Bactrox Hypoxic Chamber, supplied by Sheldon Manufacturing, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Light microscopy images of clinical S. aureus isolate <t>A1</t> cultured in SSF without Fg (SSF1) and SSF with Fg (SSF2) under aerobic ( A ), microaerophilic (3% O 2 ) ( B ), and anaerobic ( C ) conditions, evaluated at four different timepoints. The bars represent the average number ( n = 3) of CFUs recovered after conventional biofilm disruption for biofilms grown in SSF1 (blue) and SSF2 (light gray) or after biofilm disruption using trypsin (0.25%) after growth in SSF2 (dark gray). Error bars indicate standard deviation; black line indicates the inoculum of 5 × 10 7 CFU/mL. * P < 0.05, ** P < 0.01, *** P < 0.001. ns, not significant ( P ≥ 0.05).
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    hypoxic chamber bactrox  (Sheldon Manufacturing)
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    Light microscopy images of clinical S. aureus isolate <t>A1</t> cultured in SSF without Fg (SSF1) and SSF with Fg (SSF2) under aerobic ( A ), microaerophilic (3% O 2 ) ( B ), and anaerobic ( C ) conditions, evaluated at four different timepoints. The bars represent the average number ( n = 3) of CFUs recovered after conventional biofilm disruption for biofilms grown in SSF1 (blue) and SSF2 (light gray) or after biofilm disruption using trypsin (0.25%) after growth in SSF2 (dark gray). Error bars indicate standard deviation; black line indicates the inoculum of 5 × 10 7 CFU/mL. * P < 0.05, ** P < 0.01, *** P < 0.001. ns, not significant ( P ≥ 0.05).
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    bactrox hypoxic chamber model 388l  (Sheldon Manufacturing)
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    Light microscopy images of clinical S. aureus isolate <t>A1</t> cultured in SSF without Fg (SSF1) and SSF with Fg (SSF2) under aerobic ( A ), microaerophilic (3% O 2 ) ( B ), and anaerobic ( C ) conditions, evaluated at four different timepoints. The bars represent the average number ( n = 3) of CFUs recovered after conventional biofilm disruption for biofilms grown in SSF1 (blue) and SSF2 (light gray) or after biofilm disruption using trypsin (0.25%) after growth in SSF2 (dark gray). Error bars indicate standard deviation; black line indicates the inoculum of 5 × 10 7 CFU/mL. * P < 0.05, ** P < 0.01, *** P < 0.001. ns, not significant ( P ≥ 0.05).
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    Image Search Results


    Light microscopy images of clinical S. aureus isolate A1 cultured in SSF without Fg (SSF1) and SSF with Fg (SSF2) under aerobic ( A ), microaerophilic (3% O 2 ) ( B ), and anaerobic ( C ) conditions, evaluated at four different timepoints. The bars represent the average number ( n = 3) of CFUs recovered after conventional biofilm disruption for biofilms grown in SSF1 (blue) and SSF2 (light gray) or after biofilm disruption using trypsin (0.25%) after growth in SSF2 (dark gray). Error bars indicate standard deviation; black line indicates the inoculum of 5 × 10 7 CFU/mL. * P < 0.05, ** P < 0.01, *** P < 0.001. ns, not significant ( P ≥ 0.05).

    Journal: Microbiology Spectrum

    Article Title: A novel synthetic synovial fluid model for investigating biofilm formation and antibiotic susceptibility in prosthetic joint infections

    doi: 10.1128/spectrum.01980-24

    Figure Lengend Snippet: Light microscopy images of clinical S. aureus isolate A1 cultured in SSF without Fg (SSF1) and SSF with Fg (SSF2) under aerobic ( A ), microaerophilic (3% O 2 ) ( B ), and anaerobic ( C ) conditions, evaluated at four different timepoints. The bars represent the average number ( n = 3) of CFUs recovered after conventional biofilm disruption for biofilms grown in SSF1 (blue) and SSF2 (light gray) or after biofilm disruption using trypsin (0.25%) after growth in SSF2 (dark gray). Error bars indicate standard deviation; black line indicates the inoculum of 5 × 10 7 CFU/mL. * P < 0.05, ** P < 0.01, *** P < 0.001. ns, not significant ( P ≥ 0.05).

    Article Snippet: Optimization was done by assessing the growth of S. aureus A1 in both media after 4, 24, 48, and 72 h of incubation at 37°C under aerobic, microaerophilic (3% O 2 , 5% CO 2 , 92% N 2 ; Bactrox Hypoxic Chamber; SHEL-LAB, Cornelius, USA) and anaerobic conditions (5% H 2 , 5% CO 2 , 90% N 2 ; Bactronez-2 Anaerobic Chamber, SHEL-LAB).

    Techniques: Light Microscopy, Cell Culture, Disruption, Standard Deviation

    CLSM (left) and light microscopy (right) images of four of the staphylococci. ( A ) S. aureus A1, ( B ) S. aureus SAU060112, ( C ) S. capitis subsp. capitis CCUG 39451, and ( D ) S. epidermidis HD05-1 ST2, after 24 h of incubation in SSF2. CLSM: scale bars 50 µm; light microscopy: scale bars 200 µm. The contrast was adjusted to improve visualization. The light microscopy images shown (on the right) are the same as the ones shown in for the four strains mentioned.

    Journal: Microbiology Spectrum

    Article Title: A novel synthetic synovial fluid model for investigating biofilm formation and antibiotic susceptibility in prosthetic joint infections

    doi: 10.1128/spectrum.01980-24

    Figure Lengend Snippet: CLSM (left) and light microscopy (right) images of four of the staphylococci. ( A ) S. aureus A1, ( B ) S. aureus SAU060112, ( C ) S. capitis subsp. capitis CCUG 39451, and ( D ) S. epidermidis HD05-1 ST2, after 24 h of incubation in SSF2. CLSM: scale bars 50 µm; light microscopy: scale bars 200 µm. The contrast was adjusted to improve visualization. The light microscopy images shown (on the right) are the same as the ones shown in for the four strains mentioned.

    Article Snippet: Optimization was done by assessing the growth of S. aureus A1 in both media after 4, 24, 48, and 72 h of incubation at 37°C under aerobic, microaerophilic (3% O 2 , 5% CO 2 , 92% N 2 ; Bactrox Hypoxic Chamber; SHEL-LAB, Cornelius, USA) and anaerobic conditions (5% H 2 , 5% CO 2 , 90% N 2 ; Bactronez-2 Anaerobic Chamber, SHEL-LAB).

    Techniques: Light Microscopy, Incubation

    Overview of median MICs, MBCs, BPCs, and MBICs (in µg/mL) of the different antibiotics for the 18 clinical PJI isolates investigated <xref ref-type= a " width="100%" height="100%">

    Journal: Microbiology Spectrum

    Article Title: A novel synthetic synovial fluid model for investigating biofilm formation and antibiotic susceptibility in prosthetic joint infections

    doi: 10.1128/spectrum.01980-24

    Figure Lengend Snippet: Overview of median MICs, MBCs, BPCs, and MBICs (in µg/mL) of the different antibiotics for the 18 clinical PJI isolates investigated a

    Article Snippet: Optimization was done by assessing the growth of S. aureus A1 in both media after 4, 24, 48, and 72 h of incubation at 37°C under aerobic, microaerophilic (3% O 2 , 5% CO 2 , 92% N 2 ; Bactrox Hypoxic Chamber; SHEL-LAB, Cornelius, USA) and anaerobic conditions (5% H 2 , 5% CO 2 , 90% N 2 ; Bactronez-2 Anaerobic Chamber, SHEL-LAB).

    Techniques: